Journal:

Article Title: Altered distribution of natural killer cell subsets identified by CD56, CD27 and CD70 in primary and chronic human immunodeficiency virus-1 infection

doi: 10.1111/j.1365-2567.2007.02657.x

Figure Lengend Snippet: NK cell percentages and subset distribution in HIV-1 infection. (a) Representative dot plot showing gating of total, CD56bright and CD56dim NK cells.(b) Percentage of NK (CD56+ CD3–) cells in PBMC from healthy controls, patients with primary (PHI) and chronic (CHI) HIV-1 infection. (c) Percentages of CD56bright and (d) CD56dim NK cells were determined in healthy controls and in patients with primary (PHI) and chronic (CHI) HIV-1 infection. The boxes depict the 25th and 75th percentiles, with a line at the median and whiskers depicting the 5th and 95th percentiles.

Article Snippet: Total NK cell percentages are expressed as percentage of total lymphocytes, while NK cell subsets are expressed as percentage of total NK cells. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1 caption a7 NK cell percentages and subset distribution in HIV-1 infection. (a) Representative dot plot showing gating of total, CD56 bright and CD56 dim NK cells. (b) Percentage of NK (CD56 + CD3 – ) cells in PBMC from healthy controls, patients with primary (PHI) and chronic (CHI) HIV-1 infection. (c) Percentages of CD56 bright and (d) CD56 dim NK cells were determined in healthy controls and in patients with primary (PHI) and chronic (CHI) HIV-1 infection.

Techniques: Infection

Journal:

Article Title: Altered distribution of natural killer cell subsets identified by CD56, CD27 and CD70 in primary and chronic human immunodeficiency virus-1 infection

doi: 10.1111/j.1365-2567.2007.02657.x

Figure Lengend Snippet: Expression of CD27 and CD70 on NK cells in HIV-1 infection. Percentages of NK (CD56+ CD3–) cells in PBMC expressing (a) CD27 and (b) CD70. Percentages of CD56bright NK cells expressing (c) CD27 and (d) CD70. Percentages of CD56dim NK cells expressing (e) CD27 and (f) CD70. The boxes depict the 25th and 75th percentiles, with a line at the median and whiskers depicting the 5th and 95th percentiles.

Article Snippet: Total NK cell percentages are expressed as percentage of total lymphocytes, while NK cell subsets are expressed as percentage of total NK cells. fig ft0 fig mode=article f1 fig/graphic|fig/alternatives/graphic mode="anchored" m1 Open in a separate window Figure 1 caption a7 NK cell percentages and subset distribution in HIV-1 infection. (a) Representative dot plot showing gating of total, CD56 bright and CD56 dim NK cells. (b) Percentage of NK (CD56 + CD3 – ) cells in PBMC from healthy controls, patients with primary (PHI) and chronic (CHI) HIV-1 infection. (c) Percentages of CD56 bright and (d) CD56 dim NK cells were determined in healthy controls and in patients with primary (PHI) and chronic (CHI) HIV-1 infection.

Techniques: Expressing, Infection

Journal: bioRxiv

Article Title: Endowing Universal CAR T-cell with Immune-Evasive Properties using TALEN-Gene Editing

doi: 10.1101/2021.12.06.471451

Figure Lengend Snippet: a Total lymphocytes obtained from healthy donors (HD), newly-diagnosed ALL patients and AML patients selected at the time of diagnosis, complete remission, and in relapse/refractory status were characterized using mass cytometry. b Frequencies of NK cell subsets among total lymphocytes (left panel), frequencies of NKG2A(+) and NKG2C(+) subpopulations among total NK cell subset (middle panel), and ratio of NKG2A(+) over NKG2C(+) subpopulations (right panel) characterized by mass cytometry. For quantitative comparisons, data were analyzed using a one-way ANOVA test. c and d For each group described in a , NKG2C(-) and NKG2C(+) NK cells from each individual were exported and concatenated in order to generate consensus files. c The optimized parameters for T-distributed stochastic neighbor embedding (opt-SNE) algorithm were used to cluster NK cell populations based on CD56, NKG2A, CD158a,h, CD158b1,b2,j, CD57, NKp30, NKp46, NKG2D, DNAM-1, CD4 and CD8 expression. Density of NK cell clusters are shown for NKG2C(-) and NKG2C(+) NK cells in healthy donors and in AML patients at the time of diagnosis (left panel). Expression of markers of interest defining the different clusters are projected on opt-SNE maps (right panel). d The heatmap displays the mean frequencies of NK cell markers in NKG2C(-) and NKG2C(+) NK cells, relative to pooled NKG2C(-) and NKG2C(+) NK cells. e , Schematic showing the experimental design to investigate the susceptibility of ΔTRAC CAR123 ΔB2M and ΔTRAC CAR123 ΔB2M HLAE cells, engineered using optimal conditions, to NK cell-dependent depletion in vitro using PBMC from AML patient at diagnostic. ΔTRAC CAR123 ΔB2M and ΔTRAC CAR123 ΔB2M HLAE were thawed and reactivated using CD123 coated plates for 8 days. Reactivated cells were then co-cultivated for 1 day with PBMC from AML patients (n = 7) or healthy donors (n = 7) and then analyzed by flow cytometry to determine the absolute count of the remaining ΔTRAC CAR123 ΔB2M and ΔTRAC CAR123 ΔB2M HLAE . f, Box plots represent the ratio of ΔTRAC CAR123 ΔB2M and ΔTRAC CAR123 ΔB2M HLAE counts obtained in the presence of PBMCs over the counts obtained in the absence of PBMCs. Dotted lines indicate data obtained from the same PBMC donor. In each box plot, the central mark indicates the median, the bottom and top edges of the box indicate the interquartile range (IQR), and the whiskers represent the maximum and minimum data point. For quantitative comparisons, data were analyzed using a non-parametric Wilcoxon matched-pairs signed rank test. p -values are indicated on the figures.

Article Snippet: After thawing, cryopreserved purified human CD56 + NK cells, negatively selected (AllCells) (5×10 6 cells) were resuspended at 1×10 6 cells/mL in expansion NK MACS medium (NK MACS medium supplemented with 1% NK MACS supplement (Miltenyi Biotec), 5% human AB serum, IL-2 (500 IU/mL), and IL-15 (140 IU/mL)).

Techniques: Biomarker Discovery, Mass Cytometry, Expressing, In Vitro, Diagnostic Assay, Flow Cytometry

Journal: bioRxiv

Article Title: Endowing Universal CAR T-cell with Immune-Evasive Properties using TALEN-Gene Editing

doi: 10.1101/2021.12.06.471451

Figure Lengend Snippet: a Strategy for assessing resistance of ΔTRAC CAR123 ΔB2M HLAE T-cells to NK cells in a xenotransplantation model using hIL-15 NOG mice intravenously injected with human PBMCs. b Quantitation of human CD45 + immune cell and CD56 + NK cell engraftment in the spleen of hIL-15 NOG mice injected with human PBMCs or NK-depleted human PBMCs. c Representative flow cytometry plots showing the expression of HLA-E by ΔTRAC CAR123 ΔB2M HLAE T-cells at the time of injection and four days post intravenous injection in PBMC-engrafted hIL-15 NOG mice. d Box plots represent the mean value ± s.d. of c , depicting the percentage and absolute number of HLA-E(+) ΔTRAC CAR123 ΔB2M HLAE T-cells (n=5). On each box plot, the central mark indicates the median, the bottom and top edges of the box indicate the interquartile range (IQR), and the whiskers represent the maximum and minimum data point. Source data are provided as a Source Data file.

Article Snippet: After thawing, cryopreserved purified human CD56 + NK cells, negatively selected (AllCells) (5×10 6 cells) were resuspended at 1×10 6 cells/mL in expansion NK MACS medium (NK MACS medium supplemented with 1% NK MACS supplement (Miltenyi Biotec), 5% human AB serum, IL-2 (500 IU/mL), and IL-15 (140 IU/mL)).

Techniques: Injection, Quantitation Assay, Flow Cytometry, Expressing